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1. Incubation Period (Post-Inoculation)   **Location**: Inoculated cultivation bags are moved to a dark, well-ventilated incubation room; agricultural cultivation uses incubation racks directly in the room.   **Key Parameters by Stage**:   **Days 1–5 (Mycelium Recovery/Germination)**: Temperature 23–25°C, humidity 60–62°C; remove contaminated bags promptly to aid initial growth of symbiotic fungi (hairy/tough leather fungi) that decompose nutrients.   **Days 6–10 (Mycelium Feeding)**: Temperature adjusted to 20–22°C, humidity 62–65°C to support growth.    **Days 11–20**: Increase ventilation; temperature 16–20°C (to inhibit symbiotic fungi), humidity 62–65°C to balance mycelium ratios.    **Days 20–25**: Further increase ventilation (avoid strong winds); temperature 16–20°C, humidity 65–68°C; primordia emerge from inoculation holes with mist-like droplets.   **Days 25–30**: Mycelium fills bags; primordia swell with droplets and yellow water. Remove sealing film to start fruiting management.   2. Fruiting Body Management   **Young Fungus Stage**: After film removal, maintain 18–22°C, 85–90% humidity; reduce temp/humidity if yellow water appears. Ensure ventilation (no direct wind) and low diffuse light. Primordia reach 5–6 cm (light yellow) in 10–15 days.   **Color Veraison Stage (≈45 days post-inoculation)**: Lobes expand; temperature 21–23°C, humidity 92–98%. Provide 5–7 hours/day of ≥500 lux light (no direct sun) and stable temps to prevent contamination from water droplets. Color shifts (grayish-white → white/yellow → golden/orange-red) via pigment accumulation.   **Growing Season**: Maintain 85–90% humidity with regular ventilation. Avoid overheating; increase airflow to prevent mold at the base. High humidity increases susceptibility to pathogens (e.g., Trichoderma, Penicillium), so avoid spray systems. Instead, use methods like inverted budding, newspaper/non-woven fabric covering, or moistening floors/soil (via capillary action) for safe humidity control.   3. Harvesting and Processing   Harvest when fruiting bodies are fully expanded, golden yellow, elastic, and pre-spore ejection. Cut at the base, clean impurities, grade by size/quality, and dry (sun or controlled temperature) to reduce moisture for storage/transport.   Industry Insights   Inoculation requires golden ear fruiting body tissue blocks; pure tissue develops outward-inward, with yield varying by inoculation location—highlighting the critical symbiotic ratio between golden ear and *Lederma lucidum*.   - Two-step liquid culture seed preparation addresses ratio issues. Seed quality directly impacts yield, quality, and economic benefits, making it a key production factor.