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Sparassia latifolia Bag Cultivation
2025/8/11

Sparassia latifolia Bag Cultivation.jpg

1. **Cultivation Process**:

Similar to most commercial wood-decomposing fungi.

 2. **Cultivation Bag Preparation**     - **Culture Material Formulas**       - Formula 1: 70% pine sawdust, 28% cornstarch, 2% calcium carbonate; 60%-65% moisture, natural pH.       - Formula 2: 76% pine sawdust, 18% bran, 2% cornstarch, 1.5% sucrose, 1.5% gypsum, 1% superphosphate; 60%-65% moisture, natural pH.    

- **Mixing & Bagging**       - Sieve pine sawdust to remove impurities. Mix materials thoroughly (two-stage mixing recommended to avoid clumping; avoid three-stage mixing to prevent acidification).       - Use polypropylene bags (330mm×170mm×0.04mm); each holds 0.85-0.95kg wet material, with uniform tightness and centered inoculation holes. 336 bags per sterilization cart.

 3. **Sterilization, Cooling & Inoculation**     - **Sterilization**: Use a vacuum-assisted autoclave (13.5m×2.1m×2.6m) at 126°C for 2.5 hours immediately after bagging. Ensure cart spacing for steam circulation; cover bags with waterproof sheets (50mm gap from upper layer).     - **Cooling**: Spray cooling chamber with chlorine disinfectant 3 hours before sterilization ends. Cool bags to below 24°C via filtered forced ventilation.     - **Inoculation**: Use 50-55-day-old solid spawn (24 bags per bottle). Disinfect tools/hands; flame spoons. Remove 1-2cm outer spawn, wipe bottle mouth with alcohol. Two people work quickly to cover holes/surfaces with spawn; keep bag openings open <5 seconds. Re-disinfect tools/hands with 75% alcohol after each bottle; flame spoons for ≥20 seconds.  

4. **Bag Cultivation Stage Management**     - Incubate in dark at 24-26°C; mycelia germinate in 2-3 days (minimize air circulation to prevent contamination; keep floor dry).     - After 10-15 days (mycelia cover bag surface), maintain 23-25°C with ventilation. Later, lower to 22-24°C and increase ventilation. Mycelia fully colonize bags in 50-55 days.

 5. **Fruiting Stage Management**     - After primordia form, open bags and place on racks. Maintain 18-20°C, 90%-95% humidity (ultrasonic humidifiers).     - Provide 8 hours/day LED light (preferably at night to save energy). Increase ventilation to keep CO₂ at ~0.2%; monitor mushroom moisture.

 6. **Common Issues**     - Strict light/ventilation needed for primordia induction; poor conditions hinder success.     - Inadequate ventilation (high CO₂) causes deformed fruiting bodies.  

7. **Harvesting**     - Induce primordia with 20-22°C and diffuse light after mycelia colonize bags. Primordia develop into fruiting bodies in 25-30 days.     - Harvest when leaves turn pale yellow and petals unfold wavily. Stop humidification 12 hours before harvest.

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