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oculation tools

         Common inoculation tools include inoculation needles, hooks, shovels, 

hoes, rakes, rings, tweezers, scalpels, punchers, inoculation steel pipes, crucible pliers, 

hemostatic forceps, etc. Common tools for mother strain inoculation are inoculation hooks, 

shovels, hoes, scalpels, and punchers; inoculation tools can be purchased online or homemade, 

often using bicycle spokes, cut with a cutter, and then polished with sandpaper. Inoculation steel pipes 

and crucible pliers are mainly used for shaking bottle strains to transfer to mobile tanks; t

weezers or hemostatic forceps are used for filter bottle strains to transfer to fixed tanks.

Equipment and instruments

         Common equipment and instruments include distilled water generators,

 pure water machines, vertical autoclaves (small sterilizing pots), electric blast drying ovens, 

clean workbenches, biochemical incubators, constant temperature oscillators (shaking tables), 

strain storage boxes, microscopes, magnetic stirrers, induction cookers, microwave ovens, etc.

 Purified water is often used in the production of liquid culture; distilled water is used to prepare 

buffer solutions or clean probes; microscopes are used to regularly observe the morphology of mycelium.