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Common problems in the production of Morchella wheat grain strains

 1. Slow growth rate

Reason: insufficient looseness of the filler; low pH; fine material; excessive sterilization

 2. The bottom of the bag is not easy to grow

Reason: insufficient ventilation. High loading; no central inoculation hole; 

dense mycelium or blocked inoculation hole.

3. Penicillium grows at the bottom of the plastic plug

Reason: long culture time, easy growth after the wheat grain material is dry; 

poor culture environment, poor filterability of the plastic plug4. Many sclerotia

4. Many sclerotia

Reason: long culture time; more light exposure.

5. Neurospora contamination

Reason: lax aseptic operation; high culture temperature