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Oyster Mushroom Spawn Simplified & Structured
2026/5/12

Oyster mushrooms (3).png

1.What is Mushroom Spawn

Spawn is a combination of pure oyster mushroom mycelium + nutrient medium, just like seeds for crops.

High-quality spawn is the key to stable yield and good profits. Good spawn plus scientific management 

makes cultivation easier and more efficient; poor spawn cannot bring ideal harvests even with good planting skills.

2. Three-level Classification of Oyster Mushroom Spawn

Oyster mushroom adopts three-stage expanded cultivation:Mother Spawn → Original Spawn → Cultivation

 SpawnStep-by-step inoculation and expansion to increase spawn quantity and help mycelium adapt to

 growing medium.

3. Mother Spawn

Cultivated in glass test tubes with agar medium.Pure mycelium isolated from oyster mushroom fruiting body.

Mainly used for spawn preservation and later expanded breeding.Too many subcultures will cause spawn 

degeneration, so subculture times should be controlled.

4. Original Spawn (Secondary Spawn)

Inoculate mother spawn into medium like sawdust, cottonseed hulls, wheat grains.Usually cultured in

 glass/plastic spawn bottles.1 tube of mother spawn can inoculate about 5 bottles of original spawn.

Can also be cultivated in liquid medium or plastic bags.

5. Cultivation Spawn (Tertiary / Production Spawn)

Inoculate original spawn into similar nutrient medium for further expansion.Packed in plastic bags, 

directly used to inoculate oyster mushroom growing bags.Cannot be expanded again for further breeding, 

otherwise mycelium vitality and yield will drop.1 bottle of original spawn can inoculate about 20 bags of 

cultivation spawn.



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