
Classified according to the treatment of the culture medium:
(1) Fermented Substrate Cultivation: This method involves cultivating edible mushrooms after the culture
medium has undergone composting and fermentation. Fermented substrate cultivation is a method between
raw material cultivation and sterilized substrate cultivation, also known as semi-raw material cultivation.
The advantages of this method are simple operation, labor-saving, and fuel-saving; the disadvantage is
that the mushrooms are prone to disease and pest infestation in the later stages of fruiting.
(2) Sterilized Substrate Cultivation: This method uses culture medium that has been sterilized by high-pressure
or atmospheric pressure sterilization to cultivate edible mushrooms. The advantages of this method are high yield,
good quality, and sustained production; the disadvantages are that it is labor-intensive and fuel-intensive.
(3) Raw Material Cultivation: This method involves cultivating edible mushrooms by directly inoculating the
culture medium without fermentation or high-temperature sterilization. The advantages of this method are
simple operation, labor-saving, and fuel-saving, making it easily accepted by mushroom growers;
the disadvantages are low yield, insufficient sustained production, and a high risk of contamination by
miscellaneous bacteria, especially in the later stages of fruiting.
(4) Semi-Sterilized Substrate Cultivation for Oyster Mushrooms: This method involves stacking the mixed
raw materials for 2-3 days, then sterilizing them with 100°C high-temperature steam at atmospheric pressure
for 2-3 hours to kill most pathogenic bacteria and insect eggs in the culture medium. The sterilized culture
medium is then bagged while still hot, cooled, and then inoculated for cultivation.








