News

1. Cooling Procedure

Confirm the cooling environment - Confirm the sterilization procedure - Open the cabinet door - Confirm the temperature - Remove the cabinet - Arrange as required - Close the cabinet door - Confirm equipment operation - Confirm the material temperature before inoculation

2. Cooling Procedures

1. Confirm that the cooling chamber temperature is set at 14-15.5°C, the evaporator fan frequency is set, and the environment is clean and sterile.

2. Confirm that the sterilization procedure is complete.

3. Close the interlayer door.

4. For autoclaves that have completed the sterilization cycle, open the cabinet door according to the procedure.

5. Release heat to allow the hot air inside the cabinet door to dissipate through the interlayer.

6. After 15 minutes, confirm the air temperature.

7. Remove the cabinet when the temperature is below 80°C.

8. Remove the cabinet from the front to the back, following the wind direction (perpendicular to the evaporator fan direction).

9. Once cooling chamber 1 is full, place the materials in cooling chamber 2 in order, starting with the 28°C cooling chamber.

10. Close the isolation doors of each area and cool. 11. Confirm the cooling chamber pressure, temperature settings, and equipment operation.

12. Check the material temperature before inoculation to determine whether it meets inoculation requirements.

13. After inoculation, thoroughly disinfect the cooling room, buffer room, and mezzanine.

3. Precautions

① Before opening the cabinet door, check that the sterilization process is complete.

② Avoid high-temperature burns when opening the cabinet door.

③ When unloading the cabinet, confirm that the cooling chamber's cleanliness meets the requirements.

④ After unloading the cabinet, carefully check the placement of the materials.

⑤ Confirm that the equipment is operating properly.

⑥ Confirm that the temperature settings are normal.

⑦ Keep relevant records: "Cooling Chamber Temperature Record Sheet," "Cooling Chamber Evaporator Fan Frequency Conversion Record Sheet," and "Inoculation Temperature Test Record Sheet."

4. Cooling Process Standards

① Unloading Method: Above 80°C; directly into the cooling chamber (without passing through non-clean areas).

② Maintain cleanliness inside and outside the cooling chamber (clean area).

③ Forced and rapid cooling.

④ Cool the material temperature to 17-20°C to prevent high temperatures from inhibiting mycelial growth. 5. Cooling Room Hygiene Standards

After the cooling room is cleaned, first mop it with clean water, then mop it with TCCA (trichloroisocyanuric acid) disinfectant. Wipe all walls and ceilings with 250-500mg/kg of TCCA. Complete the cleaning process with a spray disinfectant and then use ultraviolet light for disinfection. Note the following:

① Follow the sanitation standards three times: sweep out garbage, clean with clean water, and disinfect with disinfectant.

② Empty the baskets and clean them promptly after unloading.

③ Ensure that the number of bacterial colonies in the environment is less than 3.

④ Choose the right disinfectant and alternate between TCCA and Sanitizer.