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1. Key Considerations in Selecting Fermentation Equipment  - **Fermentation Tank**: A core device with various types classified by operation (batch/continuous), sterilization (internal/external), stirring (mechanical/air), control (automated/manual), mobility (mobile/fixed), structure (vertical/horizontal, single-layer/sandwich). Selection depends on production process and enterprise actual conditions.   - **Air Purification System**: Critical supporting equipment with key points:    - Oil-free air compressor (reciprocating/screw type) with backup unit to avoid production shutdown losses.    - Cold dryer to cool compressed air below dew point for effective oil-water separation.    - Multi-stage high-efficiency filter (end filtration requirement: 0.01μm) plus a small flying saucer filter on the fermentation tank inlet pipe for enhanced protection.    - Temperature control device to adjust air temperature entering the tank.  

2. Fermentation Tank Cleaning and Inspection  - **Cleaning**: Thoroughly clean with running water after each use or before reuse to remove bacterial pellets, liquid, and dirt. Use a long-handled iron brush for adhered dirt; drain via the bottom inoculation valve. Remove air inlet pipe nozzles to discharge large bacterial clumps if needed.   - **Cleaning Standards**: No debris/hanging matter on inner wall, no residual bacterial pellets, and clear, contaminant-free drainage water.   - **Inspection**: After cleaning, add water to cover the heating tube (prevent dry heating), start the equipment, and check the control cabinet, heating tube, and valves for leaks. Proceed only if all pass.  

3. Tank Boiling (Sterilization of Fermentation Tank)   - **Situations Requiring Boiling**: New tanks (first use), contaminated tanks, product type changes, or long-unused tanks.   - **Steps**:    ① Close bottom inoculation and air inlet valves; add water from the feed inlet to the sight glass center line; close and tighten the feed inlet cap, and close the exhaust port.    ② Power on, press the sterilize button; heating indicator lights up.    ③ When temperature reaches 100℃, release cold air by slightly opening the exhaust valve until sterilization ends.    ④ At 123℃, the control cabinet automatically times (35 minutes). After alarming, press the alarm stop button, close the exhaust valve. After 20 minutes, open the exhaust valve, inoculation port, and air inlet to drain water, completing the process.   - **Concurrent Operations**: Sterilize materials, filter elements, and inoculation guns during tank boiling.  

4. Preparation of Liquid Culture Medium  - **Recipe** (per 1000ml water): 100g potato, 15g brown sugar, 10g glucose, 40g wheat bran, 2.0g peptone, 2.0g potassium dihydrogen phosphate, 1.0g magnesium sulfate, vitamin B1 tablets, 0.3ml sodium phosphate; pH 6.8~7.0. Add 0.02% edible vegetable oil (e.g., soybean oil) or 0.03% defoaming agent (polypropylene oxide glycerol ether) to prevent excessive foaming.    - Adjust viscosity with cornstarch (high viscosity → small mycelial pellets, which disperse well in solid medium and promote rapid fruiting).   - **Material Selection**: Fresh, mold-free potatoes (no rot/sprouts/greening); large, fresh, mold-free wheat bran (sieve fine bran with 15-20 mesh); bottled analytical/chemical grade potassium dihydrogen phosphate and magnesium sulfate.   - **Preparation**: Peel and slice potatoes (0.3-0.5cm), boil with water; add soaked wheat bran, boil 30 minutes; filter with 4 layers of wet wrung gauze; add brown sugar, glucose, KH₂PO₄, peptone, MgSO₄, vitamin B₁ sequentially; dissolve over low heat; add water to 1000ml.